Anne M. Wertheimer, PhD

Research Assistant Professor, College of Medicine, Department of Medicine, Division of Geriatrics
Research Assistant Professor, Geriatics and Immunobiology

Societies

  • American Association for the Advancement of Science
  • American Society for Microbiology
  • Gerontological Society of America
  • American Aging Association

Ongoing Research Support

N01 AI 50027-03 (Nikolich-Zugich, PI) 09/30/05-09/29/10

  • NIH/NIAID
  • Immunological basis of age-related susceptibility to West Nile virus
  • Goal: To use a succession of rodent, primate and human models to elucidate critical age related defects in innate and adaptive responses to WNV.
  • Role: Project Manger

Completed Research Support

Liver Scholar Award  Wertheimer (PI)  7/1/02-6/31/05

  • American Liver Foundation
  • Effect of hepatitis C Virus (HCV) on dendritic cell immunobiology
  • The study tested the hypothesis that HCV-derived proteins may interfere with antigen presentation. Specifically, 1) how is gene expression within the dendritic cell affected by the presence of virally derived HCV proteins? And 2) how is the antigen presentation function of dendritic cells affected by the presence of HCV proteins?
  • Role: PI

Veterans Administration  Wertheimer (PI)  4/1/99-3/31/04

  • Research Enhancement Award Program, REAP
  • Pilot: ” Interference with T cell recognition by HCV-derived proteins”
  • Specific Aims: 1)Determine if expression of HCV genes (using recombinant vaccinia virus vectors) leads to diminished cell surface expression of molecules required for recognition by CD4+ and CD8+ T lymphocytes; 2) Determine if expression of HCV genes (using recombinant vaccinia vectors) interferes with the antigen-specific recognition by CD4+ and CD8+ T cell clones.

RFA DK-01-007  Rosen (PI)  7/1/01-6/30/05

  • NIH/NIDDK
  • Cellular Immune Mechanisms of Resistance to HCV Therapy
  • The major goals were to investigate the Th1/Th2 paradigm in chronically infected HCV-positive patients undergoing antiviral therapy, how functional T lymphocyte responses are modified by therapy (change in magnitude and specificity), and to address the underlying immune mechanisms of impaired responsiveness to antiviral therapy in HCV-infected African-Americans.
  • Role: Co-investigator

R01-DK60590-01  Rosen (PI) 12/1/01-12/30/05

  • NIH/NIDDK 
  • Immune Responses in Acute Hepatitis C Infection 
  • The major goals were to investigate the CD4+ and CD8+ T cell response to HCV early after infection, to map T cell determinants, and define the role of CTL mutational escape as a mechanism of viral persistence. 
  • Role: Co-investigator
Education: 
  • BS: Michigan State University, Microbiology 1984-1988
  • PhD: Oregon Health Sciences University, Microbiology/Immunology 1992-1997
  • Post Doc: Abbott Laboratories,Virology/Immunology 1997–1998
  • Post Doc: Oregon Health & Science University, Gastrontology/Hepatology/Immunology 2000–2002
Honors and Awards: 
  • 1984: State of Michigan Competitive Scholarship, Michigan State University, East Lansing, MI
  • 1984: Academic Excellence, Michigan State University, East Lansing, MI.
  • 1984: American Association of University Women Scholarship, Michigan State University,East Lansing, MI.
  • 1993: Tarter Fellowship Award, competitive award, MRF Foundation, Portland, OR.
  • 1994: Dean's Award, Oregon Health Sciences University, Portland, OR.
  • 1994: Sears Memorial Fellowship Award, Excellence in Graduate Medical Education, Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, OR.
  • 1995-97: Pathogenesis Training Fellowship (NIH), Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, OR.
  • 1996: Dean's Award, competitive award for travel to scientific conference, Oregon Health Sciences University, Portland, OR.
  • 1997: ASM Sustaining Member Student Travel Grant. 97th National ASM meeting, Miami FL.
  • 2002: International HCV and Related Viruses Travel Award
  • 2002-2005: American Liver Foundation Liver Scholar Award
Research Interest: 

Immunobiology:  microbe-host interactions, specifically microbial pathogenesis and concomitant immune response with respect to host age.

Publications: 
  1. Tolmasky, M.E., Wertheimer A.M., L.A. Actis, and J.H. Crosa. "Characterization of the Vibrio anguillarum fur Gene: Role in Regulation of Expression of the FatA Outer Membrane Protein and Catechols." Journal of Bacteriology 176 (1994): 213-220.
  2. Wertheimer, A.M., M.E. Tolmasky, L.A. Actis, and J.H. Crosa. "Structural and Functional Analyses of Mutant Fur Proteins with Impaired Regulatory Function." Journal of Bacteriology 176 (1994): 5116-5122.
  3. Qian C.,Wertheimer A.M., M. E. Tolmasky, and J.H. Crosa. "The AngR protein and the siderophore anguibactin positively regulate the expression of iron-transport genes in Vibrio anguillarum. " Molecular Microbiology. 22 (1996): 127-134.
  4. Wertheimer, A.M., W. Verweij, Q. Chen, L.M. Crosa, M. Nagasawa, M.E. Tolmasky, L.A. Actis, and J.H. Crosa.  "Characterization of the angR gene of Vibrio anguillarum: essential role in virulence." Infection and Immunity 67 (1999): 6496-6509.
  5. Di Lorenzo, M., M. Stork, M.E. Tolmasky, L.A. Actis, D.F. Farrell, T.J. Welch, L.M .Crosa, A.M. Wertheimer, Q. Chen, P. Salinas, L. Waldbeser, and J.H Crosa. “Complete Sequence of Virulence Plasmid pJM1 from the Marine Fish Pathogen Vibrio anguillarum Strain  775.” Journal of Bacteriology. 185 (2003):5822-5830.
  6. Wertheimer,A.M., C. Miner, D.M. Lewinsohn A.W. Sasaki , E. Kaufman and H.R Rosen. “Novel CD4+ and CD8+ T-Cell Determinants Within the NS3 Protein in Subjects With Spontaneously Resolved HCV Infection.”  Hepatology 37 (2003): 577-589.
  7. Wertheimer, A.M., H.R. Rosen. “Immunity and Persistence in Hepatitis C Virus Infection.” Current Hepatitis Reports 2003, 2:152-158.
  8. Wertheimer, A.M., A. Bakke, and H.R.Rosen. “Direct Enumeration and Functional Assessment of Circulating Dendritc Cells in Patients with Liver Disease.” Hepatology  40 (2004): 335–345.
  9. Rosen HR, Hinrichs DJ, Leistikow RL, Callender G, Wertheimer AM, Nishimura MI, Lewinsohn DM, Cutting edge: Identification of hepatitis C virus-specific CD8+ T cells restricted by donor HLA alleles following liver transplantation. J Immunol. 2004 Nov 1;173(9):5355-9.
  10. Weston, S.J., R.L.Leistikow, R.K.Reddy, M.Torres, A.M. Wertheimer, D.M..Lewinsohn, S.Chou, M.P.Davey, C.Coreless, C. O’Farrelly, D.R.Nelson., and H.R. Rosen. “ Reconstitution of Hepatitis C Virus Specific T Cell Mediated Immunity Following Liver Transplantation. Hepatology 2005;41:72–81 http://www.ncbi.nlm.nih.gov/pubmed/15939788?ordinalpos=4&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum
  11. Tester I, Smyk-Pearson S, Wang P, Wertheimer A, Yao E, Lewinsohn DM, Tavis JE, Rosen HR. Immune evasion versus recovery after acute hepatitis Cvirus infection from a shared source.  J Exp Med. 2005 Jun 6;201(11):1725-31.
  12. Wertheimer, A.M., S.JPolyak, R.Leistikow, and H.R. Rosen Engulfment of apoptotic cells expressing HCV proteins leads to differential chemokine expression and STAT signaling in human dendritic cells. Hepatology. 2007 Jun;45(6):1422-32. Erratum in: Hepatology. 2007 Aug;46(2):610.
  13. Lee JA, Spidlen J, Boyce K, Cai J, Crosbie N, Dalphin M, Furlong J, Gasparetto M, Goldberg M, Goralczyk EM, Hyun B, Jansen K, Kollmann T, Kong M, Leif R, McWeeney S, Moloshok TD, Moore W, Nolan G, Nolan J, Nikolich-Zugich J, Parrish D, Purcell B, Qian Y, Selvaraj B, Smith C, Tchuvatkina O, Wertheimer A, Wilkinson P, Wilson C, Wood J, Zigon R, The International Society for Analytical Cytology Data Standards Task Force, Scheuermann RH, Brinkman RR*.  MIFlowCyt: The Minimum Information About a Flow Cytometry Experiment.  Cytometry A. Oct; 73 (10) :926-30, 2008.